raybetAPP’s fully validated highly sensitive method enables completion of thyroid hormone tests in accordance with OECD guidelines

普林斯顿，NJ，美国 - Envigo很raybetAPP高兴地宣布它已经开发并验证了一种稳健的分析方法，以评估大鼠F1后代对甲状腺激素水平的潜在影响。Envigo可以使用超高压液相色谱法偶联到高端raybetAPP串联质谱仪（UHPLC-MS / MS）进行检测。结果，经合组织生殖毒理学指南OECD 421,422和443，其包括评估甲状腺功能，现在可以在Envigo下进行。raybetAPP

This highly sensitive method was successfully validated for the determination of T3 (5 to 1,500 pg/mL) and T4 (70 to 70,000 pg/mL) in rat serum for F1 animals at days 4 and 13 of age using a sample volume of 50 µL. Envigo is also able to perform detailed microscopic examination of the thyroid glands from F1 offspring at day 13. In some instances, an evaluation of the potential effects on Thyroid Stimulating Hormone (TSH) levels in plasma for offspring at days 4 and 13 can also be undertaken using an immunoassay method.

Prior to this development, Envigo performed a PTU (Propylthiouracil) positive control developmental thyroid study as required by US Environmental Protection Agency (EPA). Quantifiable levels of TSH were detected in the plasma of pregnant female rats and their fetuses. In addition, quantifiable levels of T3 and T4 hormones were also detected in pregnant females, but the validated immunoassay technique was unable to detect T3 and T4 in foetuses. It was later shown that this was also the case for F1 offspring at days 4 and 13 of age. A substantial increase in sensitivity for detection of T3 and T4 levels was clearly required for F1 offspring before these hormones could be detected in control animals. Consequently, Envigo developed and validated the UHPLC-MS/MS technique to overcome this serious challenge and thereby enable all relevant OECD and US EPA testing requirements to be met.

Emea（欧洲，中东和亚洲）在Envigo的行动中举行的John Handley评论：“在Envigo，我们在临时临界测定中引发了对科学和技术挑战的解决raybetAPP方案。我们热衷于在甲状腺激素测定的发展方面对甲状腺激素测定进行突出作用，因此非常高兴地使我们提供的高灵敏度UHPLC-MS / MS方法。“